Double-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.
نویسندگان
چکیده
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 11 6 شماره
صفحات -
تاریخ انتشار 1977